hidi formamide (Thermo Fisher)

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Thermo Fisher hidi formamide
Hidi Formamide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hidi+formamide/TRIS-HCL/10__5194_slash_soil___11___735___2025-168-17-19
Average 99 stars, based on 1 article reviews

hidi formamide - by Bioz Stars, 2026-09

99/100 stars

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Electrophoresis:

Article Title: Updated Structure of CNBP Repeat Expansions in Patients With Myotonic Dystrophy Type 2 and Its Implication for Standard Diagnostics
Article Snippet: .. After dilution with HiDi Formamide (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA) and the addition of GeneScan 500 LIZ dye Size Standard (Applied Biosystems), capillary electrophoresis was performed on ABI Prism Genetic Analyzer 3730 (Applied Biosystems) with an injection time of 5 seconds. .. The results were assessed using GeneMarker software v2.4.0 (SoftGenetics, State College, PA).

Injection:

Gradient Centrifugation:

Article Title: Aeration and mineral composition of soil mediate microbial CUE
Article Snippet: .. 250 ng) was subjected to isopycnic gradient centrifugation in a solution of caesium trifluoroacetate (CsTFA, GE Healthcare), HiDi formamide (Thermo Fisher Scientific), and buffer (0.1 mol Tris-HCl at pH 8.0, 0.1 mol KCl, and 1 mM EDTA). .. Gradients were prepared in Ultracrimp 6 mL tubes (Thermo Scientific) and centrifuged at 130000× g using a TV-1665 Sorval Rotor (Thermo Scientific) for ≥ 65 h. Fractionation was done by piercing the tube close to the top and bottom and injecting RNAse-free water (Carl Roth) into the top using an automatic syringe pump (NE-300; New Era Pump System Inc.) Fractions were collected every 20 s https://doi.org/10.5194/soil-11-735-2025 SOIL, 11, 735–753, 2025 (300 μL each).

Incubation:

Article Title: Structural basis of RNAPII transcription on the nucleosome containing histone variant H2A.B.
Article Snippet: After the incubation, 0.04 units (for dsDNA) or 0.05 units (for nucleosomes) of DNase I (Fujifilm Wako) were added to the transcription reaction mixture and further incubated at 30 °C for 10 min. .. The samples were deproteinized by proteinase K (Roche), incubated with HiDi Formamide (Thermo Fisher Scientific) at 95 °C, and subjected to denaturing 8% polyacrylamide gel electrophoresis. ..

Article Title: Structural basis of RNAPII transcription on the nucleosome containing histone variant H2A.B.
Article Snippet: .. The samples were then deproteinized with proteinase K (Roche), incubated with HiDi Formamide (Thermo Fisher Scientific) at 95 °C to denature the RNA transcripts, and subjected to denaturing 10% polyacrylamide gel electrophoresis. .. The DY647 signal on the gel was detected using a Typhoon imager (Cytiva).

Polyacrylamide Gel Electrophoresis:

Sequencing:

Article Title: Blow Flies Pose a Public Health Hazard near Urban Combined Sewer Overflows.
Article Snippet: Over 700 municipalities in the United States have combined sewer systems.. Following rainfall events, flows to wastewater treatment plants (WWTPs) may exceed capacity, resulting in billions of gallons of wastewater being discharged annually.. In Indianapolis, Indiana, precipitation events of ≥6 mm result in up to 90 sewage overflow events annually.

Amplification:

Article Title: Genetic diversity and intercontinental dispersal of temperate and subarctic populations of Dibothriocephalus dendriticus (Cestoda; Diphyllobothriidea), a causative agent of dibothriocephalosis.
Article Snippet: .. For fragment analysis, 1 ll of the amplified PCR product was mixed with 8.5 ll of HiDi formamide and 0.5 ll of GeneScan-LIZ 500 Size Standard (Thermo Fisher Scientific). .. The mixture was then denatured for 5 min at 95 °C and capillary electrophoresis was performed on the 3500 Genetic Analyzer (Thermo Fisher Scientific).

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